Posts Tagged ‘culture’
The overall objective of the proposed project is to establish a platform for more robust fermentation processes and production organisms by understanding and controlling heterogeneity. It is essential to optimise fermentation parameters for achieving the most efficient production process. In most research projects on this topic, the population of microorganisms was considered homogeneous. However, research has shown that a typical population of microorganisms in a fermentation is heterogeneous. Due to continued technological developments in different fields, for example in genetics and molecular biology (reporter systems), flow cytometry and microfluidics (micro-bioreactors), we have now finally reached a phase where the investigation of the effect of cultivation parameters on the heterogeneity of a microbial population has become possible, which is the focus of this project.
The central project hypothesis is that there exists an optimum level of heterogeneity leading to a robust fermentation process with sustained high productivity. To investigate this hypothesis, reporter systems for cell growth and productivity will be constructed for industrially relevant model organisms (E. coli and Saccharomyces cerevisiae), which will allow to obtain a distribution of these properties for the population, e.g. by using flow cytometry. The effect of cultivation parameters on these properties will be investigated via experiments in continuous culture micro-bioreactors. Both physiological adaptations to the signals it perceives in the culture, and genetic change allowing selection of optimally adapted forms to conditions in the culture will be researched. Experimental results at microscale will be extrapolated to labscale and pilotscale.
The position is for 2 years and funded through a recently established international network project “POPCORN”. The project will be carried out at Section for Microbiology, Departmenet of Biology, University of Copenhagen. The position is to be filled as soon as possible.
Qualifications
We are looking for a skilled and competent molecular biologist with experience in bacterial activity detection at single cell level.
• Ph.D. in microbiology, molecular biology or a similar degree
• Documented experience within FACS.
• Documented experience with fluorescent reporter gene technology in bacteria.
• It is preferred that the candidate has a good knowledge on activity staining technologies
• Documented experience within several gene-cloning techniques and molecular biology techniques in general.
• You will be able to cooperate well with your colleagues and enjoy both teamwork and cross-functional cooperation.
For further information, contact: Professor Søren J. Sørensen sjs@bio.ku.dk. Read the rest of this entry »
Applications are invited for a three year studentship to study the role of LINE-1 repetitive elements in the development of colorectal and breast cancer. The project will utilise molecular biology, biochemical and tissue culture techniques to investigate why LINE-1 become active in cancer cells and to determine whether their activation is sufficient to promote cell invasiveness. The successful student will be based at the University of Nottingham’s Medical School in Derby and will be expected to spend some time at the School of Biomedical Sciences in University Park, Nottingham. Further details on the group’s research are available at:
http://www.nottingham.ac.uk/GEM/Research/surgery.aspx or
http://www.nottingham.ac.uk/genetics/people/tufarelli/index.php.
Students should have a first or upper second class honours degree in a relevant biological science (or equivalent), and they will be expected to register for a PhD. Some laboratory experience is desirable.
This studentship is expected to start in October 2010 and a stipend at the current University rate will be provided. Due to funding restrictions, this studentship is only open to UK/EU students.
Informal enquiries may be addressed to either Dr C Tufarelli, tel: 01332 724 645, Email: cristina.tufarelli@nottingham.ac.uk or Mr J Lund, tel: 01332 724 703, Email: Jon.Lund@nottingham.ac.uk. Read the rest of this entry »
The McNeil Center will appoint a recent recipient of the PhD as a Postdoctoral Fellow for a two-year term beginning 1 July 2011. The fellow will receive a starting stipend of at least $41,000; health insurance; private office space in the Center’s building at the northeastern gateway to the University of Pennsylvania’s historic campus; library, computer, and other privileges at the university; and access to the Philadelphia area’s magnificent manuscript, rare book and museum collections. Modest funds for travel and research are available. During the two-year term of appointment, the fellow will teach two courses in an appropriate department at the University of Pennsylvania. All McNeil Center fellows are expected to be in residence during the academic year and to participate in the Center’s program of seminars and other activities.
The remainder of the fellow’s time will be devoted to research and writing. While this fellowship is particularly appropriate for projects designed to turn a doctoral dissertation into a publishable monograph, any project dealing with the histories and cultures of North America in the Atlantic world before 1850 will be considered. Proposals dependent on the use of Philadelphia-area archives and libraries are particularly welcome. During or after the term of appointment, the fellow may submit his or her manuscript for consideration by the Center’s Early American Studies series, published by the University of Pennsylvania Press, but will be under no obligation to do so. Read the rest of this entry »
